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Unless explicitly stated the mice were sacrificed 16 h after the CS exposure via cardiac exanguastion and bronchoalveolar lavage (BAL) was performed using two 750 L rinses of cold isotonic potassium phosphate solution
Phase A, Open III, Label
blood work may be advised
Samples were tested for the presence of AIs (2-androstenol (5-androst-2-en-17-ol), 2-androstenone (5-androst-2-en-17-one), 3-androstenol (5-androst-3-en-17-ol), 3-androstenone (5-androst-3-en-17-one), 4-androstene-3,6,17 trione (6-oxo), aminoglutethimide, anastrozole, androsta-1,4,6-triene-3,17-dione (androstatrienedione), androsta-3,5-diene-7,17-dione (arimistane), exemestane, formestane, letrozole, and testolactone), and anti-estrogenic substance (bazedoxifene, clomiphene, cyclofenil, fulvestrant, ospemifene, raloxifene, tamoxifen, and toremifene) prohibited by WADA
The concerns are formulation-level: copper ions can potentially destabilize some retinoid formulations, and layering both in one application step can increase irritation risk in sensitive users
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