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Clearing method for 3-dimensional immunofluorescence of osteoarthritic subchondral human bone reveals peripheral cholinergic nerves

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The eight stereoisomers are RRR, SRR , RSR , RRS , RSS , SSR , SRS , and SSS

Mice were observed throughout the duration of each experiment for signs of mortality and morbidity, and overall appearance (activity, general response to handling, touch, ruffled fur)